Date of Award:

5-1-1983

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Biology

Department name when degree awarded

Life Sciences:Biology

Committee Chair(s)

David B. Drown

Committee

David B. Drown

Committee

Bill B. Barnett

Committee

Robert W. Sidwell

Abstract

The purpose of this study was to determine and compare the rates of inactivation by chlorine of reovirus, rotavirus, and poliovirus at pH 6 and pH 10. The inactivation rates at pH 6 were greater than those at pH 10. Hypochlorous acid was found to be more effective than hypochlorite ion in the inactivation of all three viruses. The efficiency of inactivation of reovirus and rotavirus increased as the concentration of hypochlorite ion was increased. Conversely, the efficiency of inactivation decreased as the concentration of hypochlorous acid was increased. Infectious reovirus was inactivated at a faster rate than the potentially infectious reovirus. The degree of resistance of the potentially infectious reovirus decreased as the concentration of chlorine was increased. No significant differences in inactivation rates were noted between the infectious and potentially infectious rotavirus. At both pH 6 and pH 10, poliovirus was the most resistant to inactivation by chlorine followed by potentially infectious reovirus. The infectious reovirus and both forms of rotavirus were nearly identical in inactivation rates and were the least resistant to inactivation.

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