Date of Award:
5-1-1983
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Department name when degree awarded
Life Sciences:Biology
Committee Chair(s)
Jon Y. Takemoto
Committee
Jon Y. Takemoto
Abstract
An Mr 27,500 membrane protein from Rhodopseudomonas sphaeroides strain 8-17, a non-photosynthetic bacteriochlorophyll-less mutant, has been reported to be the photosynthetic reaction center subunit H found in all photosynthetic isolates of R. sphaeroides. The possible occurence of reaction center polypeptide H in mutant strain 8-17 would indicate that, unlike reaction center subunits L and M, complete bacteriochlorophyll is not required for its synthesis or insertion into the membrane. In experiments reported here, however, purified reaction center subunit H differed from the mutant 8-17 polypeptide in net charge, mobility in sodium dodecyl sulfate-polyacrylamide gels, and 125I-labelled peptide map patterns. Further, aerobic culture conditions which inhibit the expression of reaction center subunit H in the wild type strain do not affect the synthesis of the 8-17 polypeptide. In summary, the data indicate that mutant strain 8-17 does not synthesize the reaction center H subunit and that reaction center H subunit synthesis is linked to the synthesis of complete bacteriochlorophyll.
Recommended Citation
Gillies, Kevin, "Absence of Photosynthetic Reaction Center Polypeptide H in Rhodopseudomonas sphaeroides" (1983). Biology. 502.
https://digitalcommons.usu.edu/etd_biology/502
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