Date of Award:
5-1-1984
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Department name when degree awarded
Life Sciences:Biology
Committee Chair(s)
Jon Y. Takemoto
Committee
Jon Y. Takemoto
Committee
Fred Post
Committee
John Simmons
Abstract
The purpose of this work was to construct a DNA vector containing the Escherichia coli lactose operon and devise a method for the subsequent introduction into Rhodopseudomonas sphaeroides. Expertise in methods for the purification and manipulation of DNA was developed and improvements upon methods were devised. PRSF 1010 proved to be a poor cloning vector due to a lack of selectable resistance markers and useable restriction sites. The lac operon was cloned into pBR 325 using either HindIII or BamHI restriction endonucleases. Restriction maps of the resulting plasmids were constructed. Attempts to transform R. sphaeroides with plasmid DNA by established protocols for other bacteria were unsuccessful. A procedure is outlined by which a lac operon containing plasmid can be introduced into R. sphaeroides via conjugation with E. coli.
Recommended Citation
Stevenson, Brian, "Genetic Manipulation of Rhodopseudomonas sphaeroides for Lactose Utilization" (1984). Biology. 520.
https://digitalcommons.usu.edu/etd_biology/520
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