Date of Award:

5-1-1985

Document Type:

Dissertation

Degree Name:

Doctor of Philosophy (PhD)

Department:

Biology

Department name when degree awarded

Biology

Committee Chair(s)

Gene W. Miller

Committee

Gene W. Miller

Abstract

The exposure of isolated chloroplast and tonoplast vesicles to solutions containing soluble fluoride compounds results in reduced rates of photochemical activity. These reductions can be related to fluoride's effects on the chemistry of these structures. In the chloroplast, photophosphorylation was inhibited. This inhibition was due to binding of fluoride to the chloroplast ATPase (CF1). Other major functions (Calvin cycle, PSI, PSII, NADP+ reductase) were not affected. The tonoplast was inhibited in two ways. The tonoplast ATPase shows decreases in activity at high fluoride concentrations above 30 mM. At concentrations lower than 30 mM the ATPase is not affected but sealed tonoplast vesicle ΔpH formation is inhibited. It is postulated that fluoride does not allow the dissipation of Δ��by anion movement. This allows a rapid build up of a membrane potential which then inhibits further ΔpH buildup and ATP hydrolysis. I believe that F- binds in a competitive manner to a c1- channel in the tonoplast membrane, preventing c1- movement due to H+ ion pumping.

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