Date of Award:
5-1-1986
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Department name when degree awarded
Biology
Committee Chair(s)
Joseph K. K. Li
Committee
Joseph K. K. Li
Committee
F. J. Post
Committee
J. Y. Takemoto
Abstract
The genetic relatedness of the prototype bluetongue viruses isolated in the United States were examined by RNA/RNA hybridization. Genomic dsRNAs of the five United States prototype bluetongue viruses were separated by either SOS-PAGE or NuSieve agarose gel electrophoresis. These were blotted by both standard Northern technique and a recently developed, rapid alkali-blotting method to positively charged nylon membranes. The blotted RNAs were then probed with 3'-end labeled dsRNA of each serotype. Initially, single segments were individually hybridized to identify cognate genes. Total genomic dsRNAs were subsequently probed to determine genetic relatedness. The genes coding for the nonstructural proteins NS1 and NS2 were the most conserved. Most of the RNA segments coding for the core proteins were also well conserved, with segment S1 showing some diversity among the five serotypes. The segments coding for the outer capsid proteins appear to have a wide degree of sequence divergence, with segment L2 having little or no cross hybridization among the five serotypes.
Recommended Citation
Kowalik, Timothy F., "The Genetic Relatedness of United States Prototype Bluetongue Viruses by RNA/RNA Hybridization" (1986). Biology. 543.
https://digitalcommons.usu.edu/etd_biology/543
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