Date of Award:

5-1-1988

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Biology

Department name when degree awarded

Biology

Committee Chair(s)

Anne J. Anderson

Committee

Anne J. Anderson

Committee

John Carman

Committee

John R. Simmons

Abstract

Culture filtrate and mycelial surface components of the ectomycorrhizal fungus Rhizopogon vinicolor were partially purified by ion exchange and gel filtration chromatography and characterized. These Rhizopogon vinicolor components induced accumulations of condensed phenolics in treated conifer and benn calli and bean cotyledons. The treated issues became visibly brown relative to control tissues. The accumulation of phenolics in plant tissues suggests that certain components from the fungus possessed elicitor activity. Elicitor activity on treated been cotyledons was quantified by measurement of accumulations of low molecular weight and condensed phenolics. Data quantifying elicitor activity with intact conifer seedling roots and conifer calli were inconclusive because the methods were inappropriate. Further research is required for assessment of a role for elicitors in the development of incompatibility observed in distinct patterns of specificity in certain ectomycorrhizal interactions.

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