Date of Award:
5-1-1997
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Committee Chair(s)
Dana K. Vaughan
Committee
Dana K. Vaughan
Committee
Peter C. Ruben
Committee
Greg Podgorski
Abstract
Immunolabelling of phosphorylated and nonphosphorylated neurofilaments in teleost retinal horizontal cells, which are nonspiking neurons, is described. Light microscopy (postembedding immunolabelling) of retinal tissue sections revealed nonphosphorylated neurofilaments localized to regions where horizontal cell axon terminals, dendrites, and somata are located. In contrast, phosphoneurofilaments were localized to regions where axons and axon terminals of various retinal neurons, including horizontal cells, are found, as in typical spiking central nervous system neurons. Electron microscopy (preembedding immunolabelling) demonstrated that phosphoneurofilaments were localized to cone horizontal cell axons and axon terminals. Immunolabelling of primary cell cultures, however, revealed that axon-specific distribution of phosphoneurofilaments in cone horizontal cells was lost; phosphoneurofilaments were found throughout the neuron, despite regeneration of an axon-like neurite. It is unknown if this phosphorylation of somatodendritic neurofilaments is a consequence or a cause of the loss of neuronal polarity induced by dissociation procedures, which typically remove horizontal cell axons and dendrites.
Recommended Citation
Cole, David Dale, "Polar Distribution of Neurofilaments in a Nonspiking Central Nervous System Neuron" (1997). Biology. 637.
https://digitalcommons.usu.edu/etd_biology/637
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