Date of Award:

5-1-1997

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Biology

Committee Chair(s)

Dana K. Vaughan

Committee

Dana K. Vaughan

Committee

Edmund D. Brodie Jr.

Committee

William Brindley

Abstract

To identify HNK-1 bearing molecules, anti-HNK-1 was used to probe adult hybrid bass retina, including mature, central retina and the circumferential germinal margin (CGM), where new cells are constantly added to the neural circuitry. The HNK-1 antigen was heavily distributed in both of the plexiform (synaptic) layers, in tufts of Müller cell microvilli at the outer limiting membrane, throughout the optic fiber layer, and in tubular processes surrounding cone horizontal cells in the inner nuclear layer. A differential fixation scheme suggested that the Müller cell microvillar and tubular process HNK-1 immunoreactivity was due primarily to glycoproteins, with the remainder possibly due to glycolipids. Western blotting revealed significant HNK-1 immunoreactivity in protein bands at 200k, 189k, 148k, 130k, and 85k. The overall HNK-1 antigen distribution seen in adult bass retina strongly suggests a role for HNK-1 in axon fasciculation, synaptogenesis, and in as-yet uncharacterized relationships between cone horizontal cells.

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