Date of Award:
5-1-1997
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Committee Chair(s)
Dana K. Vaughan
Committee
Dana K. Vaughan
Committee
Jon Y. Takemoto
Committee
Peter C. Ruben
Abstract
The morphologies of intact retina and acutely isolated retinal neurons are described for a relatively newcomer model for studies of visual physiology, the hybrid bass (Morone chrysops x M. saxatilus). These features are indistinguishable from the white bass (M. chrysops) at the light microscopic level. In situ immunolocalization patterns for several glial and neuronal markers also match previous work in the white bass. Carbonic anhydrase is found in retinal layers corresponding to Müller (glial) cells and horizontal cells; glial fibrillary acidic protein (GFAP) and glutamine synthetase are found in Müller cells only; and the intermediate M, subunit of the neurofilament triplet protein, NF160, is found in layers surrounding cone horizontal cell bodies only. When hybrid bass retinas are acutely dissociated and the resulting isolated cells are immunolabelled for the same four markers, the results confirm the interpretations from in situ experiments. Moreover, when cells are cultured for periods of 5-15 days, immunolabelling patterns do not change. In particular, the cell adhesion carbohydrate epitope, HNK-1, is preferentially expressed on type 1 cone horizontal cells, which are extending new neurites, and all cone horizontal cell types continue to express carbonic anhydrase. The cytochemical similarities between Müller (glial) cells and horizontal cells are discussed.
Recommended Citation
Ki, Kyeongsuk, "Hybrid Bass Retinal Cells In Situ and In Vitro: Structure and Immunocytochemistry" (1997). Biology. 641.
https://digitalcommons.usu.edu/etd_biology/641
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