Date of Award:

5-1-2005

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Biology

Committee Chair(s)

Carol D. von Dohlen

Committee

Carol D. von Dohlen

Committee

Paul G. Wolf

Committee

Karen E. Mock

Abstract

Phragmatopoma californica (found in the Pacific Ocean) and P. caudata (found in the Atlantic Ocean) are intertidal, marine reef-building polychaete worms that have been found to produce viable larvae in a laboratory setting, but are otherwise geographically isolated. The first study was aimed at determining their specific status by analyzing the DNA sequences of cytochrome c oxidase subunit I (COI) and the first internal transcribed spacer region (ITS-1). Phylogenetic analyses revealed that P. californica individuals shared no COI haplotypes or ITS-1 sequences with P. caudata individuals and formed well supported separate clades. The molecular data therefore supports the status of P. californica and P. caudata as separate species. A second study using principal components analysis and dendrograms produced from the Amplified Fragment Length Polymorphisms data from P. californica populations, along with analysis of the COI haplotypes, suggested that there was no detectable population structure correlated with geography in this species.

Included in

Biology Commons

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