Ultrastructural Evidence for Temperature-Dependent Ca2+ Release from Fish Sarcoplasmic Reticulum During Rigor Mortis

Authors

Hideki Ushio
Shugo Watabe
Muneaki Iwamoto
Kanehisa Hashimoto

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

Abstract

The release or leakage of ca2+ from the sarcoplasmic reticulum (SR) during rigor mortis of fish muscle was investigated by transmission electron microscopy using pyroantimonate and related biochemical changes.

Ca2+ -pyroantimonate deposits were observed in the SR immediately after spiking the fish. At the onset of rigor for fish stored at 0°C, no deposits were found in the SR; however, fish stored for the same period at woe which were still in the pre-rigor state, clearly showed Ca2+ deposits in the SR.

In association with the Ca2+ translocation, ATP degraded faster at 0 c than at 10 °C, probably due to enhancement of myofibrillar ATPase activity by the increasing Ca2 + concentrations.

Therefore, rapid Ca2+ release from the SR at 0°C seemed to trigger the acceleration of fish rigor mort is at this temperature, analogous to the phenomenon called "cold shortening."

Recommended Citation

Ushio, Hideki; Watabe, Shugo; Iwamoto, Muneaki; and Hashimoto, Kanehisa (1991) "Ultrastructural Evidence for Temperature-Dependent Ca2+ Release from Fish Sarcoplasmic Reticulum During Rigor Mortis," Food Structure: Vol. 10: No. 3, Article 9.
Available at: https://digitalcommons.usu.edu/foodmicrostructure/vol10/iss3/9