Date of Award
Master of Science (MS)
Biological and Irrigation Engineering
H. Scott Hinton
Ronald C. Sims
Charles D. Miller
The objective of this study is to optimize the growth media and genes to maximize PHB production computer simulations, and to evaluate the simulation results through experiments. In order to achieve this objective, the metabolism of E. coli that produces PHB was modeled using the existing E. coli genome-scale metabolic networks. To identify the best additional nutrient for PHB production, carbon sources, minerals, amiano acids, nitrogen sources, and sulfur sources were examined by production potential analysis. Strain design was conducted to find the unwanted reactions to overexpress PHB synthesis pathway.
Heshiki, Yoshitaro, "Optimization of Polyhydroxybutyrate Production in Recombinant Escherichia Coli Through Metabolic Modeling and Simulation" (2013). All Graduate Plan B and other Reports. 291.
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