Design of a multiplex, surface-enhanced Raman scattering immunoassay for detection and diagnosis of hematological malignancies

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Elizabeth Vargis


The detection and diagnosis of hematological malignancies (blood cancers) require the specific identification of multiple cell surface markers called cluster of differentiation (CD) markers. In order to quantify CD markers traditional immunoassays such as ELISA, and fluoresces immunoassays have been developed. Some of these traditional immunoassays can detect multiple proteins at the same time (this is called immunoassay multiplexing). Traditional multiplex immunoassays' require extensive incubation time, and multiple washing steps resulting in total assay run times often in excess of 4-6 hours, and a reduction in assay sensitivity due to non-specific binding. In this research a multiplex surface-enhanced Raman scattering (SERS) immunoassay has been designed for the detection of multiple CD markers in solution. The assay uses labeled magnetic nanoparticles for separation of the individually bound proteins, and labeled SERS nanoparticles for quantification. The quantification of multiple CD markers is achieved through spectral multiplexing, and an appropriate spectral deconvolution method.

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