Investigation on the Chemoresistance of Breast Cancer Cells with Breast Cancer Metastasis Suppressor1 (BRMS1) by Raman Microspectroscopy and Atomic Force Microscopy (AFM)


Qifei LiFollow

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Breast cancer metastasis suppressor 1 (BRMS1) is a protein mainly distributed around cellular nucleus that can suppress the metastasis of cancer without affecting the growth of the original cancer. Nuclear factor-kappa B (NF-jB) activity and AKT phosphorylation are associated with BRMS1 implicated in chemoresistance. In this study, Atomic force microscopy (AFM) was to differentiate the biomechanical properties and Raman microspectroscopy was used to compare the biochemical differences among various cell types (A549, 231, 231/BRMS1, 435, and 435/BRMS1) treated with anti-cancer drug doxorubicin (DOX), which mainly interacted with cellular nucleus. The results from AFM illustrated that Young's modulus and adhesion force of breast cancer cells with BRMS1 decreased after anti-cancer drug DOX exposure, while the Young's modulus and adhesion force of breast cancer cells without BRMS1 increased after DOX incubation. Principal component analysis exhibited that five cell types can be easily differentiated after 4h DOX exposure. However, Raman peak intensity changes of different cancer cell lines were very similar with the presence of bands corresponding to DNA/RNA, lipids and proteins, reflecting that all groups reacted to DOX consistently, indicating that BRMS1 has no effect on cellular chemoresistance within 4h.

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