2nd International Triticeae Symposium
Richard R-C. Wang
The steps involved in the transfer of alien genetic variation will be outlined and the impact of recent technologies on improving the efficiency of the process will be discussed. The selection of parents is the first step; it is critically important that each selection be carefully screened for maximum expression of the desired trait. The crossing process is becoming increasingly more efficient with improved efficiency of growth regulators and embryo rescue media. Doubled haploid methods are being used to facilitate the production of chromosome addition lines. Molecular methods such as RFLPs, RAPDs, chromosome banding, and in situ hybridization add an increased level of resolution to the identification of chromosome additions and the monitoring of introgressed chromosome segments. Emerging technologies such as monocot transformation, chromosome-specific libraries, and transposon tagging may soon replace some of the traditional methods of gene transfer.
Fedak, George; O'Donoughue, L.; and Armstrong, K. C., "Procedures for Transfer of Agronomic Traits from Alien Species to Crop Plants" (1994). Herbarium Publications. Paper 16.