Date of Award

2013

Degree Type

Thesis

Degree Name

Departmental Honors

Department

Animal, Dairy, and Veterinary Sciences

First Advisor

Lee Rickords

Abstract

Using commercially available antibodies, we created a modified ELISA strip assay to determine pregnancy in cattle. The anti‐progesterone antibody was printed on small membrane strips via an Inkjet printer. The strips were subsequently analyzed using varying concentrations of progesterone. The objective of this study was to assess this novel antibody printing process and to determine the binding activity of the anti‐progesterone antibody. The long‐term objective is to develop a pen-side pregnancy test that would cost less than $1.00. In this specific study, it was determined that the anti‐progesterone antibody used did not provide adequate sensitivity for visualization required for pen‐side analysis. Additional studies will assess the sensitivity of other anti‐progesterone antibodies and the possible implementation of amplification steps.

Share

COinS