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Depredation of domestic livestock by wildlife is a leading source of human–wildlife conflict, often requiring intervention at the local level. Historically, these interventions have resulted in the use of lethal methods to remove the offending animal. In response to increased public opposition to lethal control methods, wildlife managers have sought to identify effective nonlethal biological options to mitigate wildlife depredations. In 2018, we tested the concept of a biological deterrent using red wolf (Canis rufus) scat that had historically been spread along fence lines to prevent depredation of lambs (Ovis aries) and kid goats (Capra aegagrus hircus) at the North Carolina State University College of Veterinary Medicine 32-ha Teaching Animal Unit (TAU), North Carolina, USA. To conduct the study, we deployed paired camera traps at 3 locations where we had previously observed coyotes (C. latrans) accessing the TAU. The study was conducted over a 94-day period alternating between no scat and freshly collected scat that was placed every 3 days from adult male red wolves. The study period overlapped lambing and kidding season. In addition to coyotes, the camera traps routinely detected red foxes (Vulpes vulpes) and raccoons (Procyon lotor). The red wolf scat we placed at the access point did not deter any of the mesocarnivores from entering the pasture.