The present study was conducted to investigate the knowledge and understanding of Universal Newborn Hearing Screening (UNHS) in nursing professionals. A group of 15 adult, licensed nurses with varied professional experience participated in the study. Participants completed both objective and subjective measurements to evaluate their current knowledge of training procedures. A free online UNHS training program offered through the National Center for Hearing Assessment and Management (NCHAM) served as both the objective measure and training module. Participants completed pre- and post-surveys as the subjective measure of their UNHS training. Results of these surveys were compared across the participants to determine significant outcomes. Results of this study suggest nursing professionals are not confident in their current level of training concerning UNHS. Additionally, the participants' scores on the online training module testing showed improvement from pre- to post-testing.