This paper reviews the work undertaken on the X-ray microanalysis of growth cartilage particularly in relation to matrix calcification. Attention is focused on the methodology available to accurately localize and retain the elements of interest. A method is described which involves rapid freezing, low temperature freeze drying in a custom built device, and embedding in Spurr resin. The results show that little tissue damage occurs and that elements of physiological interest are accurately localized at the sub-cellular level. Where damage occurs to chondrocytes as a result of freezing, however, dense intra mitochondrial granules are seen suggesting a phase transformation takes place resulting in the precipitation of calcium phosphate. Further improvements may be made in retaining the more labile elements such as K by using resins which polymerize at low temperature.
"X-Ray Microanalysis of Growth Cartilage After Rapid Freezing, Low Temperature Freeze Drying and Embedding in Resin,"
Scanning Microscopy: Vol. 1
, Article 28.
Available at: https://digitalcommons.usu.edu/microscopy/vol1/iss3/28