Digital Image Processing of Electron Micrographs: The PIC System II

Authors

Benes L. Trus, National Institutes of Health
Michael Unser, National Institutes of Health
Thierry Pun, National Institutes of Health
Alasdair C. Steven, National Institutes of Health

Abstract

The PIC system, an integrated package of Fortran programs and subroutines designed to run on the Digital Equipment Corporation VAX family of computers, has been developed for analysis of electron micrographs with emphasis on the particular requirements for structural analysis of biological macromolecules. The substantially improved VAX version of PIC reported here has been developed from an earlier PDP-11 version which was, in turn, developed from a set of IBM 370 programs called MDPP. PIC now encompasses over 150 commands or processing operations that afford a comprehensive range of image processing operations including image restoration, enhancement, Fourier analysis, correlation averaging, and multivariate statistical analysis including clustering and classification. In particular, we describe our software for correction of imperfect lattices, as well as programs for correlation alignment and averaging of "single particle" images.

Recommended Citation

Trus, Benes L.; Unser, Michael; Pun, Thierry; and Steven, Alasdair C. (1992) "Digital Image Processing of Electron Micrographs: The PIC System II," Scanning Microscopy: Vol. 1992: No. 6, Article 40.
Available at: https://digitalcommons.usu.edu/microscopy/vol1992/iss6/40