This paper describes the details of quantitative electron probe microanalysis (EPMA) performed with a wavelength dispersive spectrometer (WDS). EPMA was carried out on the giant neuron of a fresh frozen ganglion from the snail Lymnaea stagnalis. The freeze-dried cryosections were compared with sections of freeze-dried, embedded tissue. It was found, that in the ganglion there are two kinds of neurons with a different chlorine concentration of 11 mmole/liter and 32 mmole/liter. Isolated neurons in culture were shown to differ in elemental composition from those in the ganglion tissue.
Pogorelov, A.; Pogorelova, V.; Repin, N. V.; and Mizin, I.
"Quantitative Biological Electron Probe Microanalysis with a Wavelength Dispersive Spectrometer,"
Scanning Microscopy: Vol. 1994
, Article 8.
Available at: https://digitalcommons.usu.edu/microscopy/vol1994/iss8/8