Confocal Scanning Laser Microscopy (CSLM) is particularly well suited for the acquisition of 3-dimensional data of microscopic objects. In the CSLM a specific volume in the object is sampled during the imaging process and the result is stored in a digital computer as a three-dimensional memory array. Optimal use of these data requires both the development of effective visual representations as well as analysis methods. In addition to the well known stereoscopic representation method a number of alternatives for various purposes are presented. When rendering in terms of solid-looking or semitransparent objects is required, an algorithm based on a simulated process of excitation and fluorescence is very suitable. Graphic techniques can be used to examine the 3-dimensional shape of surfaces. For (near-)real time applications a representation method should not require extensive previous data-processing or analysis.
From the very extensive field of 3-D image analysis two examples are given.
Brakenhoff, G. J.; van der Voort, H. T. M.; Baarslag, M. W.; Mans, B.; Oud, J. L.; Zwart, R.; and van Driel, R.
"Visualization and Analysis Techniques for Three Dimensional Information Acquired by Confocal Microscopy,"
Scanning Microscopy: Vol. 2
, Article 2.
Available at: https://digitalcommons.usu.edu/microscopy/vol2/iss4/2