Effect of X-Irradiation on Adenylate Cyclase Activity and Cyclic AMP Content of Primary Human Fibroblasts

Authors

Z. Somosy, "Frederic Joliot-Curie" National Research Institute for Radiobiology and Radiohygiene
A. Takáts, "Frederic Joliot-Curie" National Research Institute for Radiobiology and Radiohygiene
Tamara Kubasova, "Frederic Joliot-Curie" National Research Institute for Radiobiology and Radiohygiene
F. Sudár, National Institute of Oncology
G. J. Köteles, "Frederic Joliot-Curie" National Research Institute for Radiobiology and Radiohygiene

Abstract

Ionizing radiation provokes an increase of the cAMP level in several organs and body fluids. After reviewing the relevant literature we present the results of our own experiments on primary human fibroblasts. X-irradiation at doses of 0.5 and 2.5 Gy in vitro evoked a rapid and reversible increase of adenylate cyclase enzyme activity. A significant increase in cAMP level of these cells was also observed.

Adenylate cyclase was usually localized basolaterally on the surface of unirradiated cells, while irradiation resulted in a modification of distribution, i.e., the enzyme activity also appeared in apical localization.

Recommended Citation

Somosy, Z.; Takáts, A.; Kubasova, Tamara; Sudár, F.; and Köteles, G. J. (1988) "Effect of X-Irradiation on Adenylate Cyclase Activity and Cyclic AMP Content of Primary Human Fibroblasts," Scanning Microscopy: Vol. 2: No. 4, Article 31.
Available at: https://digitalcommons.usu.edu/microscopy/vol2/iss4/31