Restored teeth were extracted from test animals at four time intervals (1 hour, 1 day, 1 week, and 3 months) following amalgam insertion. Extracted teeth were frozen in liquid nitrogen, cryo-fractured so as to expose the pulps and then freeze-dried. Pulps were analyzed for mercury content by energy dispersive spectrometry (EDS) and atomic absorption spectrophotometry (AAS). Mercury levels appeared below the detection limits of EDS but could be detected by AAS which showed the highest readings seven days after amalgam insertion.
Lentz, D. L.; Buchanan, J. T.; and Basinger, D. R.
"Analyses of Dental Pulp in Restored Teeth,"
Scanning Microscopy: Vol. 3
, Article 21.
Available at: https://digitalcommons.usu.edu/microscopy/vol3/iss1/21