Scanning Microscopy


Quantitative microanalysis has been performed with a JAMP 10 Scanning Auger Microscope at 0.5 μm spot size, on carpospores of Gracilaria secundata Harv.. Samples were fixed in glutaraldehyde and osmium tetroxide and embedded in plastic material. Thin sections were placed on solid copper discs. the surfaces of which had been smoothed by electropolishing.

Quantification of the nitrogen content was performed using a standard with known content of nitrogen.

Nitrogen was localized in the nucleus, chloroplasts and osmiophilic inclusions of the carpospore. Trace amounts of nitrogen were also found in cell walls, starch grains and in the embedding material.

The use of Scanning Auger Microscopy on biological material is discussed.

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