Radiation doses of 20, 50 or 100 Gy caused the same time related decrease for RNA and proteoglycan (PG) synthesis in embryonic cartilage in vitro (4 days culture). In this paper, participation of lysosomes in this radiation response is investigated. Therefore, we employ a cytochemical method using 𝜷-glycerophosphate as substrate for acid phosphatase (AP) detection. Increase of AP was found 2 days after irradiation and increased during the whole culture period. The increase was more pronounced with a higher radiation dose. Stimulation of AP activity explains the observed radiation response of RNA and PG synthesis.
Cornelissen, M. and de Ridder, L.
"Dose- and Time-Dependent Increase of Lysosomal Enzymes in Embryonic Cartilage in Vitro after Ionizing Radiation,"
Scanning Microscopy: Vol. 4
, Article 22.
Available at: https://digitalcommons.usu.edu/microscopy/vol4/iss3/22