The presence of mucus and/or cellular debris can obscure the fine morphology of the gastrointestinal or respiratory luminal surface, when observed by scanning electron microscopy. With the intent of obtaining a good cleaning of the mucosal surface without altering the ultrafine morphology of epithelial cells, a new model of sonicator/ultrasonicator is presented.
The instrument is supplied with a control system for wave frequency, amplitude and form, and permits a precise regulation of the wave energy. With this instrument it is possible to produce a "cleaning effect" by using any kind of frequency (either sonic or ultrasonic) and/or amplitude and/or waveform and/or liquid.
We report the application of sonic frequencies through water as a fluid for immersion to obtain a gentle and slow removing of mucus and in order to explore the possibility to clean hydrated tissues.
With the employment of sonic frequencies (from 5 to 15 kHz modulated by 200 Hz) and water as the immersion fluid, we were able to generate a gentle wave energy which effected an optimal removal of the mucus, with the consequent exposure of a well preserved epithelial surface of rat trachea and small intestine.
Familiari, G.; Familiari, A.; Macchiarelli, G.; DiDio, L. J. A.; and Motta, P. M.
"The Use of Sonic Frequencies as a Cleaning Agent of Specimens to be Observed by Scanning Electron Microscopy,"
Scanning Microscopy: Vol. 7
, Article 12.
Available at: https://digitalcommons.usu.edu/microscopy/vol7/iss1/12