Technical Improvements in Corrosion Casting of Small Specimens: A Study on Mesonephric Tubules and Vessels of Chicken Embryos

Authors

A. Carretero, Autonomous University of Barcelona
H. Ditrich, University of Vienna
M. Navarro, Autonomous University of Barcelona
H. Splechtna, University of Vienna
J. Ruberte, Autonomous University of Barcelona

Abstract

The injection technique for corrosion casting of small, embryonic material can be improved by using a "chemical ligature" (cyanocrylate). With this simple method, leakage of the resin at the injection site is prevented and the mechanical stability of the cannula-vessel coupling is improved.

The blood vascular system of chicken embryos as small as Hamburger-Hamilton stage 24 (approximately 4.5 days of incubation) has successfully been injected using this procedure. Corrosion casts of the mesonephric tubular system have been made in a similar manner. Additionally, a simple way for secure transport of the fragile casts by immersion in 10% gelatin is suggested.

Recommended Citation

Carretero, A.; Ditrich, H.; Navarro, M.; Splechtna, H.; and Ruberte, J. (1993) "Technical Improvements in Corrosion Casting of Small Specimens: A Study on Mesonephric Tubules and Vessels of Chicken Embryos," Scanning Microscopy: Vol. 7: No. 4, Article 22.
Available at: https://digitalcommons.usu.edu/microscopy/vol7/iss4/22