The injection technique for corrosion casting of small, embryonic material can be improved by using a "chemical ligature" (cyanocrylate). With this simple method, leakage of the resin at the injection site is prevented and the mechanical stability of the cannula-vessel coupling is improved.
The blood vascular system of chicken embryos as small as Hamburger-Hamilton stage 24 (approximately 4.5 days of incubation) has successfully been injected using this procedure. Corrosion casts of the mesonephric tubular system have been made in a similar manner. Additionally, a simple way for secure transport of the fragile casts by immersion in 10% gelatin is suggested.
Carretero, A.; Ditrich, H.; Navarro, M.; Splechtna, H.; and Ruberte, J.
"Technical Improvements in Corrosion Casting of Small Specimens: A Study on Mesonephric Tubules and Vessels of Chicken Embryos,"
Scanning Microscopy: Vol. 7
, Article 22.
Available at: https://digitalcommons.usu.edu/microscopy/vol7/iss4/22