The most sensitive analytical techniques available today for detecting immuno assay complexes are radio or enzyme immuno analytical techniques, by which quantities of 107-108 analyte molecules can be detected. With the introduction of scanning force microscopy, a new method for detecting biological processes became available. Here, we examine the feasibility of using scanning force microscopy as a biosensitive tool. We demonstrate that single or multiple rabbit anti-human serum albumin molecules form complexes with preadsorbed single human serum albumin molecules on mica. However, no interaction is observed between human immunoglobulin G molecules and preadsorbed single albumin molecules; only separate antigens and antibodies are observed at random positions on the mica. This shows the ability of scanning force microscopy to act as a biosensor for detection of immunocomplexes, and to act as a very powerful tool to study molecule-surface interactions in general.
Quist, Arjan P.; Bergman, Anna A.; Reimann, Curt T.; Oscarsson, Sven O.; and Sundqvist, Bo U. R.
"Imaging of Single Antigens, Antibodies, and Specific Immunocomplex Formation by Scanning Force Microscopy,"
Scanning Microscopy: Vol. 9
, Article 8.
Available at: https://digitalcommons.usu.edu/microscopy/vol9/iss2/8