Multiplex B-Cell Cancer Marker Detection Using Surface-Enhanced Raman Spectroscopy
Class
Article
Department
Biological and Irrigation Engineering
Faculty Mentor
Elizabeth Vargis
Presentation Type
Oral Presentation
Abstract
Surface-enhanced Raman spectroscopy is a light scattering technique that shows great promise for the detection of multiple biomarkers in the same sample (multiplexing). This is of particular importance for the diagnosis and treatment of B-cell cancers. In order to properly treat these cancers a cell surface marker profile must be obtained through protein multiplexing. This profile is used by a pathologist to identify the cancer type and provide the optimal treatment. In order to use surface-enhanced Raman spectroscopy for multiplex biomarker analysis, spectacularly unique gold nanoparticle probes have been designed for the targeting and optical detection of cell surface proteins. These probes have very narrow spectral profiles and as a result are the ideal platform for the detection of multiple biomarkers. Through conjugation of antibodies to these probes specific biomarker targeting has been demonstrated. This technique represents a new method for mutliplex biomarker analysis and has promising applications for the diagnosis and treatment of B-cell malignancies.
Start Date
4-9-2015 11:00 AM
Multiplex B-Cell Cancer Marker Detection Using Surface-Enhanced Raman Spectroscopy
Surface-enhanced Raman spectroscopy is a light scattering technique that shows great promise for the detection of multiple biomarkers in the same sample (multiplexing). This is of particular importance for the diagnosis and treatment of B-cell cancers. In order to properly treat these cancers a cell surface marker profile must be obtained through protein multiplexing. This profile is used by a pathologist to identify the cancer type and provide the optimal treatment. In order to use surface-enhanced Raman spectroscopy for multiplex biomarker analysis, spectacularly unique gold nanoparticle probes have been designed for the targeting and optical detection of cell surface proteins. These probes have very narrow spectral profiles and as a result are the ideal platform for the detection of multiple biomarkers. Through conjugation of antibodies to these probes specific biomarker targeting has been demonstrated. This technique represents a new method for mutliplex biomarker analysis and has promising applications for the diagnosis and treatment of B-cell malignancies.