Title of Oral/Poster Presentation

Synthetic Piriform Spider Silk

Presenter Information

Cole PetersonFollow

Class

Article

Department

Biological and Irrigation Engineering

Faculty Mentor

Randy Lewis

Presentation Type

Poster Presentation

Abstract

Spider silk from N. clavipes have a range of useful properties in addition to remarkable mechanical properties. These silks are uniquely suited for biomedical applications as they are generally biocompatible and biodegradable. Piriform silk is an adhesive protein used by the spider to adhere its web to a variety of substrates. Sequencing of mRNA extracted from the piriform silk gland of N. clavipes has revealed two unique amino acid motifs. The structural role of these motifs in the resulting piriform silk attachment disk is unknown. The specific aim of this project is to produce recombinant piriform spider silk in Escherichia coli. Three biosynthetic proteins will be produced, two based on individual amino acid motifs and one based on the overall piriform sequence. The resulting piriform-analogue proteins will be used to produce fibers, films and gels for mechanical testing. The structural role of piriform's unique motifs will be characterized using X-Ray Diffraction and Circular Dichroism on solid samples. Elucidating the mechanical and structural roles of these motifs will add to the existing repertoire of characterized motifs for the production of tunable, chimeric spider silk materials.

Start Date

4-9-2015 12:00 PM

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Apr 9th, 12:00 PM

Synthetic Piriform Spider Silk

Spider silk from N. clavipes have a range of useful properties in addition to remarkable mechanical properties. These silks are uniquely suited for biomedical applications as they are generally biocompatible and biodegradable. Piriform silk is an adhesive protein used by the spider to adhere its web to a variety of substrates. Sequencing of mRNA extracted from the piriform silk gland of N. clavipes has revealed two unique amino acid motifs. The structural role of these motifs in the resulting piriform silk attachment disk is unknown. The specific aim of this project is to produce recombinant piriform spider silk in Escherichia coli. Three biosynthetic proteins will be produced, two based on individual amino acid motifs and one based on the overall piriform sequence. The resulting piriform-analogue proteins will be used to produce fibers, films and gels for mechanical testing. The structural role of piriform's unique motifs will be characterized using X-Ray Diffraction and Circular Dichroism on solid samples. Elucidating the mechanical and structural roles of these motifs will add to the existing repertoire of characterized motifs for the production of tunable, chimeric spider silk materials.