Title of Oral/Poster Presentation

Comparing Cell Freezing Methods

Class

Article

College

College of Agriculture and Applied Sciences

Faculty Mentor

Craig Day

Presentation Type

Poster Presentation

Abstract

My project compares several different methods of freezing human cell lines for storage to see which method best preserves the cells. Cell storage is an important but often overlooked part of any lab that does in vitro testing. Freezing cells allows cell lines to be used long after they are harvested, and allows laboratories to use cells at much lower passage numbers than if they had been continuously grown since harvest. It is important to store the cells properly because poor storage methods can seriously harm or kill valuable cell lines. To test different cell freezing strategies, I will harvest stocks of Huh7 cells, a continuous cell line derived from a human liver. I will freeze the cells with different methods. Then, I will then thaw the cells and allow them to grow overnight and then count the cells to see which freezing method preserved the cells’ viability the best.

Location

The South Atrium

Start Date

4-12-2018 12:00 PM

End Date

4-12-2018 1:15 PM

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Apr 12th, 12:00 PM Apr 12th, 1:15 PM

Comparing Cell Freezing Methods

The South Atrium

My project compares several different methods of freezing human cell lines for storage to see which method best preserves the cells. Cell storage is an important but often overlooked part of any lab that does in vitro testing. Freezing cells allows cell lines to be used long after they are harvested, and allows laboratories to use cells at much lower passage numbers than if they had been continuously grown since harvest. It is important to store the cells properly because poor storage methods can seriously harm or kill valuable cell lines. To test different cell freezing strategies, I will harvest stocks of Huh7 cells, a continuous cell line derived from a human liver. I will freeze the cells with different methods. Then, I will then thaw the cells and allow them to grow overnight and then count the cells to see which freezing method preserved the cells’ viability the best.