Session

2025 Poster

Location

Brigham Young University Engineering Building, Provo, UT

Start Date

5-5-2025 9:55 AM

Description

We intend to create a vegan meat alternative by genetically modifying Hericium erinaceus (Lion's Mane mushroom) to express the soy leghemoglobin gene. This gene, commonly used in plant-based meats, contributes both color and umami flavor reminiscent of animal-derived hemoglobin. Our approach involves extracting and sequencing Lion's Mane DNA, identifying viable genomic insertion sites, and applying CRISPR-Cas9 to insert the leghemoglobin gene. Concurrently, we are optimizing lab-based cultivation methods to ensure reliable growth and propagation of both wild-type and genetically modified strains. This project integrates fungal biotechnology, genetic engineering, and sustainable food innovation.

Available for download on Wednesday, May 06, 2026

Share

COinS
 
May 5th, 9:55 AM

From Forest Floor to Future Food: Cultivating and Engineering Lion's Mane Mushrooms

Brigham Young University Engineering Building, Provo, UT

We intend to create a vegan meat alternative by genetically modifying Hericium erinaceus (Lion's Mane mushroom) to express the soy leghemoglobin gene. This gene, commonly used in plant-based meats, contributes both color and umami flavor reminiscent of animal-derived hemoglobin. Our approach involves extracting and sequencing Lion's Mane DNA, identifying viable genomic insertion sites, and applying CRISPR-Cas9 to insert the leghemoglobin gene. Concurrently, we are optimizing lab-based cultivation methods to ensure reliable growth and propagation of both wild-type and genetically modified strains. This project integrates fungal biotechnology, genetic engineering, and sustainable food innovation.