Session
2025 Poster
Location
Brigham Young University Engineering Building, Provo, UT
Start Date
5-5-2025 9:55 AM
Description
This study focuses on the construction of a plasmid harboring biphenyl (BPH) genes, with the aim of enabling potential degradation of polychlorinated biphenyls (PCBs). Optimization experiments were conducted to enhance PCR amplification and assembly efficiency. PCR conditions were refined using a primer concentration gradient and by varying magnesium chloride levels through adjustments in reaction buffer volume, resulting in improved specificity and yield. Six BPH gene fragments were successfully litigated into the pSHDY vector using Gibson Assembly, alongside a GA+ positive control. The resulting construct represents a foundational step toward engineering organisms capable of PCB biodegradation.
Cracking the Code for PCB Cleanup: PCR Optimization Meets Synthetic Biology
Brigham Young University Engineering Building, Provo, UT
This study focuses on the construction of a plasmid harboring biphenyl (BPH) genes, with the aim of enabling potential degradation of polychlorinated biphenyls (PCBs). Optimization experiments were conducted to enhance PCR amplification and assembly efficiency. PCR conditions were refined using a primer concentration gradient and by varying magnesium chloride levels through adjustments in reaction buffer volume, resulting in improved specificity and yield. Six BPH gene fragments were successfully litigated into the pSHDY vector using Gibson Assembly, alongside a GA+ positive control. The resulting construct represents a foundational step toward engineering organisms capable of PCB biodegradation.