Isolation and Characterization of Sixteen Microsatellite Loci in the Mountain Pine Beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae)

Authors

C. S. Davis, University of Alberta
Karen E. Mock, Utah State UniversityFollow
B. J. Bentz, USDA Forest Service
S. Bromilow, University of Alberta
N. V. Bartell, University of Northern British Columbia
B. W. Murray, University of Northern British Columbia
A. D. Roe, University of Alberta
J.E. K. Cooke, University of Alberta

Document Type

Article

Journal/Book Title/Conference

Molecular Ecology Resources

Volume

9

Issue

3

Publisher

Wiley-Blackwell

Publication Date

2009

First Page

1071

Last Page

1073

Abstract

We isolated 16 polymorphic microsatellite loci in the mountain pine beetle (Dendroctonus ponderosae Hopkins) and developed conditions for amplifying these markers in four multiplex reactions. Three to 14 alleles were detected per locus across two sampled populations. Observed and expected heterozygosities ranged from 0.000 to 0.902 and from 0.100 to 0.830, respectively. Three loci deviated from Hardy–Weinberg equilibrium in one sampled population. One of these loci may be sex linked. These markers will be useful in the study of population structure in this important pest species.

Comments

Originally published by Wiley-Blackwell. Publisher's PDF and HTML fulltext available through remote link.

Recommended Citation

Davis CS, Bentz BJ, Mock KE, Bromilow S, Bartell NV, Murray BW, Roe AD, Cooke JEK (2009) Isolation and characterization of sixteen microsatellite loci in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae: Scolytinae). Molecular Ecology Resources, 9, 1071-1073.