Ultrastructural Study of Bone Formation on Synthetic Hydroxyapatite in Osteoblast Cultures

Authors

J. M. Sautier, Institut Biomedical des Cordeliers, Paris
J. R. Nefussi, Universite Paris VII, Paris
N. Forest, Universite Paris VII, Paris

Abstract

Collagenase isolated rat calvaria cells forming a mineralized matrix in vitro were cultured in the presence of synthetic hydroxyapatite. Interactions between bone cells and hydroxyapatite biomaterial were followed by transmission electron microscopy. The appearance of a granular, collagen free, electron-dense layer at the periphery of the material was noted initially. Progressively, an amorphous, granular material formed and extended between the hydroxyapatite aggregates. Osteoblastic cells then synthesized an osteoid matrix which mineralized on the first formed granular collagen free layer, following a classical pattern of calcification . Demineralization of ultrathin sections confirmed the presence of this interface between the material and bone tissue formed in vitro.

Recommended Citation

Sautier, J. M.; Nefussi, J. R.; and Forest, N. (1991) "Ultrastructural Study of Bone Formation on Synthetic Hydroxyapatite in Osteoblast Cultures," Cells and Materials: Vol. 1: No. 3, Article 3.
Available at: https://digitalcommons.usu.edu/cellsandmaterials/vol1/iss3/3