Cells and Materials


Primary bovine osteoclasts were obtained by an outgrowth method from bovine periosteum and cultured for 7 days on an ionomeric cement for biomaterial testing. Osteoclasts cultured on slices of bovine bone and on glass microscope cover-slides served as a control. The cells were characterised as osteoclasts by a number of tests. Osteoclasts showed positive staining for tartrate resistant acid phosphatase and reactivity with the antibodies 13C2 and 23C6, which react with the alphachain of the vitronectin receptor. Addition of salmon calcitonin to the culture medium led to sudden cessation of lamellipodial activity. The cells resorbed bone by making pits. In mixed cultures with osteoblasts, the morphology of the osteoclasts on the smooth ionomeric cement surface was comparable to the one on glass cover-slides, revealing broad cytoplasmatic extensions on the material. Acridine orange staining demonstrated viability of cells until the end of the culture period and increased acidification after parathyroid hormone (PTH) stimulation. Scanning electron microscopy did not reveal erosion of the material by osteoclasts. No signs of aluminium toxicity on osteoclasts could be detected during the 7 day culture period, although an increased uptake of aluminium into the cell was demonstrated.