Cells and Materials


Bone chamber intravital microscopy combines the control volume of in vitro models and the chemical complexity of in vivo models to optimize the study of bone physiology in situ. As an optical tool it provides a window to dynamic events at the tissue level of magnification. In particular, it allows measures of microvascular events: (1) in space by magnifying local perfused vasculature and microcirculation at any instant, and (2) in time by providing the same volume of tissue for weekly viewing of an evolving process such as bone healing. This quartz-bearing titanium screw has revealed a consistent order for vascular-bone progression during healing. Videography and digital image processing allowed simultaneous measurement of osteogenesis, angiogenesis, blood supply and permeability.