The Growth of Fetal Human Sensory Ganglion Neurons in Culture: A Scanning Electron Microscopic Study

Authors

Seung U. Kim, University of British Columbia, Vancouver
Kookmin M. Kim, Baltimore Veterans Administration Medical Center
Giuseppe Moretto, University of British Columbia, Vancouver
James H. Kim, University of British Columbia, Vancouver

Abstract

Sensory neurons of 8-week human fetal dorsal root ganglia were dissociated into single cells by trypsinization and cultured on coverslips for 4 months, in either serum-containing or serum-free chemically defined media. At different times the cultures were fixed and prepared for scanning electron microscopy. Fetal sensory neurons in culture regenerated axons within 24 hours which were characterized by axonal growth cones at their tips and the neuronal perikarya assumed spherical or a bonnet-like morphology.

Recommended Citation

Kim, Seung U.; Kim, Kookmin M.; Moretto, Giuseppe; and Kim, James H. (1985) "The Growth of Fetal Human Sensory Ganglion Neurons in Culture: A Scanning Electron Microscopic Study," Scanning Electron Microscopy: Vol. 1985: No. 2, Article 33.
Available at: https://digitalcommons.usu.edu/electron/vol1985/iss2/33