The Quick-Freezing of Single Intact Skeletal Muscle Fibers at Known Time Intervals Following Electrical Stimulation

Authors

Rashid Nassar, Duke University and Veterans Administration Medical Centers
Nancy R. Wallace, Duke University and Veterans Administration Medical Centers
Isaiah Taylor, Duke University and Veterans Administration Medical Centers
Joachim R. Sommer, Duke University Medical Center

Abstract

Single intact frog skeletal muscle fibers quick-frozen after known time intervals following electrical stimulation are examined electron microscopically in thin sections, after freeze-substitution, in freeze-fracture/etch preparations, and in cryosections prepared for x-ray microprobe analysis. Techniques are described to perform these operations on a single fiber. Notable morphological differences between conventionally fixed and cryopreserved muscle fibers, and between fibers quick- frozen at different post-stimulation intervals are demonstrated.

Recommended Citation

Nassar, Rashid; Wallace, Nancy R.; Taylor, Isaiah; and Sommer, Joachim R. (1986) "The Quick-Freezing of Single Intact Skeletal Muscle Fibers at Known Time Intervals Following Electrical Stimulation," Scanning Electron Microscopy: Vol. 1986: No. 1, Article 33.
Available at: https://digitalcommons.usu.edu/electron/vol1986/iss1/33