Cryogenic preparation has been used to study changes that occur as a dry specimen undergoes hydration. Conventional techniques such as critical point drying or anhydrous fixation are unsuitable for studying such changes. Seed tissue, a dry moss and various foods were chosen to show dry-to-wet transitions that take place after wetting of the dry sample begins. Cryogenically prepared specimens have great potential, not only in the study of changes which occur during imbibition of one sample, but also in the study of samples with different moisture content.
Lott, John N. A. and Kerr, Patrice
"Use of Cryogenically Prepared Samples in the Scanning Electron Microscopic Study of Dry-to-Wet Transitions,"
Scanning Electron Microscopy: Vol. 1986
, Article 16.
Available at: https://digitalcommons.usu.edu/electron/vol1986/iss3/16