Analysis of the Effects of Inhaled Diesel Exhaust on the Alveolar Intravascular and Interstitial Cellular Components of Rodent Lungs

Authors

M. A. Wallace, Wayne State University
S. O. Salley, Wayne State University
M. I. Barnhart, Wayne State University

Abstract

Transmission electron microscopy (TEM) was used to determine the effect of diesel engine exhaust (DEE) on the intravascular and interstitial cellular population of the lungs of exposed rats and guinea pigs. Animals with matched controls were subjected to environments of either 250, 750, 1500 or 6000 μg/m³ for either 2 weeks, 6 weeks, 10 weeks or 18 months. These animals were sacrificed immediately following the exposure periods and their lungs perfused with fixative. Following dissection, random stratified biopsies from the lungs of these animals were made. Ultrathin sections from the alveolar lung were prepared and conventionally processed for TEM and randomly photographed to compose a micrograph database. These micrographs were analyzed by point counting using a Zeiss MOP 3 Digital Image Analyzer. The results indicated no significant intravascular cellular response but a significant increase in the mononuclear population in the interstitium.

Recommended Citation

Wallace, M. A.; Salley, S. O.; and Barnhart, M. I. (1987) "Analysis of the Effects of Inhaled Diesel Exhaust on the Alveolar Intravascular and Interstitial Cellular Components of Rodent Lungs," Scanning Microscopy: Vol. 1: No. 3, Article 52.
Available at: https://digitalcommons.usu.edu/microscopy/vol1/iss3/52