Ex vivo Platelet Deposition on Fibronectin-Preadsorbed Surfaces

Authors

William E. Collins, University of Wisconsin
Deane F. Mosher, University of Wisconsin
Anil R. Diwan, University of Wisconsin
Kedar D. Murthy, University of Wisconsin
Scott R. Simmons, University of Wisconsin
Ralph M. Albrecht, University of Wisconsin
Stuart L. Cooper, University of Wisconsin

Abstract

Temporal platelet deposition profiles of canine plasma fibronectin (CPFN) adsorbed to different polymers ex vivo and the in vitro characteristics of CPFN adsorption were studied in an attempt to correlate the two. The maximum platelet deposition (𝚪_plt^max) obtained at a protein preadsorption time of 30 min was greater than that obtained using an adsorption time of 120 min for all surfaces studied. At 30 min of preadsorption, 𝚪_plt^max was 520, 560 and 1230 platelets/1000 μm² on Biomer, polyethylene (PE) and oxidized PE (OXPE), respectively. In contrast, the platelet deposition at 120 min. of fibronectin preadsorption was about 60-90 platelets/1000 μm² on all polymers studied. The surface concentrations of adsorbed CPFN measured using ¹²⁵I-CPFN, were in the order PE> OXPE > Biomer. The adsorbed protein concentration increased with increasing adsorption time. The surface distribution of adsorbed CPFN was visualized with antibody-labelled colloidal gold and scanning electron microscopy. The extent of staining was lowest on PE, greater on Biomer, and highest on OXPE, roughly similar to the order of platelet deposition. Platelet deposition ex vivo appears to correlate with the irnmunogold-stainable-adsorbed protein rather than with the total amount of adsorbed protein.

Recommended Citation

Collins, William E.; Mosher, Deane F.; Diwan, Anil R.; Murthy, Kedar D.; Simmons, Scott R.; Albrecht, Ralph M.; and Cooper, Stuart L. (1987) "Ex vivo Platelet Deposition on Fibronectin-Preadsorbed Surfaces," Scanning Microscopy: Vol. 1: No. 4, Article 18.
Available at: https://digitalcommons.usu.edu/microscopy/vol1/iss4/18