Ionizing Radiation-Induced Apoptosis and DNA Repair in Murine Erythroleukemia Cells

Authors

R. Di Pietro, National Research Council, Italy
L. Centurione, National Research Council, Italy
E. Santavenere, National Research Council, Italy
M. A. Centurione, National Research Council, Italy
G. Sanitá Di Toppi, Istituto di Radioterapia
L. Zamai, Istituto di Anatomia Umana Normale
R. Rana, National Research Council, Italy

Abstract

A morphological study of DNA repair and apoptotic patterns in relationship with cell cycle events was performed on murine erythroleukemia cells. The presence and distribution of DNA replicon sites were evaluated through the BrdU-anti BrdU immunofluorescence and immunogold techniques in light and electron microscopy. Different patterns of labelling and percentages of BrdU positive cells were observed depending on irradiation dose (up to 60 Gy) and time in post-irradiation culture (up to 24 hours). An enlargement of the S phase of the cell cycle was evidenced 18 hours post-irradiation as determined by flow cytometry analysis. The high resolution approach showed that, in spite of several morphological alterations, BrdU labelling was present even in cells displaying early and late apoptotic features.

Recommended Citation

Di Pietro, R.; Centurione, L.; Santavenere, E.; Centurione, M. A.; Sanitá Di Toppi, G.; Zamai, L.; and Rana, R. (1996) "Ionizing Radiation-Induced Apoptosis and DNA Repair in Murine Erythroleukemia Cells," Scanning Microscopy: Vol. 10: No. 1, Article 20.
Available at: https://digitalcommons.usu.edu/microscopy/vol10/iss1/20