Quantitative Biological Electron Probe Microanalysis with a Wavelength Dispersive Spectrometer

Authors

A. Pogorelov, Institute of Theoretical and Experimental Biophysics of the Russian AcademyFollow
V. Pogorelova, Institute of Theoretical and Experimental Biophysics of the Russian Academy
N. V. Repin, Institute of Theoretical and Experimental Biophysics of the Russian Academy
I. Mizin, Institute of Theoretical and Experimental Biophysics of the Russian Academy

Abstract

This paper describes the details of quantitative electron probe microanalysis (EPMA) performed with a wavelength dispersive spectrometer (WDS). EPMA was carried out on the giant neuron of a fresh frozen ganglion from the snail Lymnaea stagnalis. The freeze-dried cryosections were compared with sections of freeze-dried, embedded tissue. It was found, that in the ganglion there are two kinds of neurons with a different chlorine concentration of 11 mmole/liter and 32 mmole/liter. Isolated neurons in culture were shown to differ in elemental composition from those in the ganglion tissue.

Recommended Citation

Pogorelov, A.; Pogorelova, V.; Repin, N. V.; and Mizin, I. (1994) "Quantitative Biological Electron Probe Microanalysis with a Wavelength Dispersive Spectrometer," Scanning Microscopy: Vol. 1994: No. 8, Article 8.
Available at: https://digitalcommons.usu.edu/microscopy/vol1994/iss8/8