Microscopic Analysis of DNA and DNA-Protein Assembly by Transmission Electron Microscopy, Scanning Tunneling Microscopy and Scanning Force Microscopy

Authors

T. Müller-Reichhert, European Molecular Biology Laboratory, GermanyFollow
H. Gross, Eidgenössische Technische Hochschule-Hönggerberg, Switzerland

Abstract

To investigate DNA and DNA-protein assembly, nucleic acids were adsorbed to freshly cleaved mica in the presence of magnesium ions. The efficiency of DNA adhesion and the distribution of the molecules on the mica surface were checked by transmission electron microscopy. In addition, various kinds of DNA-protein interactions including DNA wrapping and DNA super-coiling were analyzed using electron microscopy. In parallel, this Mg²⁺/mica method can be applied (1) to analyze embedded DNA by scanning tunneling microscopy, (2) to visualize freeze-dried, metal coated DNA-protein complexes by tunneling microscopy, and (3) to image DNA or DNA-protein interaction in air or in liquid by scanning force microscopy. An advantage of such a correlative approach is that parallel imaging can reveal complementary information. The benefit of such a combined approach in analysis of protein-induced DNA bending is discussed.

Recommended Citation

Müller-Reichhert, T. and Gross, H. (1996) "Microscopic Analysis of DNA and DNA-Protein Assembly by Transmission Electron Microscopy, Scanning Tunneling Microscopy and Scanning Force Microscopy," Scanning Microscopy: Vol. 1996: No. 10, Article 10.
Available at: https://digitalcommons.usu.edu/microscopy/vol1996/iss10/10