Freeze-Dried Human Leukocytes Stabilized with Uranyl Acetate During Low Temperature Embedding or with OsO4 Vapor After Embedding

Authors

L. Edelmann, Universität des Saarlandes
A. Ruf, Medizinisch-Diagnostisches Institut, Germany

Abstract

Two new simple stabilization procedures for freeze-dried biological material are introduced which are compatible with low temperature embedding (LTE) in Lowicryl. The first method uses a Lowicryl K11M/HM20 mixture supplemented with 0.3% uranyl acetate for LTE. For the second method polymerized Lowicryl blocks containing the freeze-dried material are exposed to OsO₄ vapor which penetrates into the Lowicryl block and stabilizes the embedded specimen. The quality of structural preservation is demonstrated with human leukocytes.

Recommended Citation

Edelmann, L. and Ruf, A. (1996) "Freeze-Dried Human Leukocytes Stabilized with Uranyl Acetate During Low Temperature Embedding or with OsO4 Vapor After Embedding," Scanning Microscopy: Vol. 1996: No. 10, Article 24.
Available at: https://digitalcommons.usu.edu/microscopy/vol1996/iss10/24