Proton Microprobe and Particle Induced X-Ray Emission (PIXE) Analysis for Studies of Pathological Brain Tissue

Authors

K. G. Malmqvist, Lund Institute of Science and Technology
A. Brun, University Hospital
K. Inamura, University Hospital
E. Martins, University Hospital
L. G. Salford, University Hospital
B. K. Siesjö, University Hospital
U. A. S. Tapper, Lund Institute of Science and Technology
K. Themner, Lund Institute of Science and Technology

Abstract

Particle Indiced X-ray Emission and proton microprobe analyses have been applied for the investigation of regional elemental distributions in connection with various pathological states in the brain. Malignant brain tumours and adjacent histologically intact tissue removed during surgery were analysed with PIXE. Systematic elemental variations, e.g., for calcium and selenium, were observed in the tumour front. The proton microprobe was applied to study the Ca and K concentrations in various cell strata in hippocampus following transient ischaemia in rat brain. Significant increases in the Ca level occurred in selectively vulnerable cells within 48 h after the ischaemia.

Recommended Citation

Malmqvist, K. G.; Brun, A.; Inamura, K.; Martins, E.; Salford, L. G.; Siesjö, B. K.; Tapper, U. A. S.; and Themner, K. (1988) "Proton Microprobe and Particle Induced X-Ray Emission (PIXE) Analysis for Studies of Pathological Brain Tissue," Scanning Microscopy: Vol. 2: No. 3, Article 42.
Available at: https://digitalcommons.usu.edu/microscopy/vol2/iss3/42