Secondary ion mass spectroscopy (SIMS) was used to obtain images representing the intracellular distribution of molecules labelled with carbon 14.
Deoxyadenosine labelled with carbon 14 was added to a cultured human fibroblast cell medium, and the intracellular distribution of this molecule was studied using three different SIMS instruments: the CAMECA IMS 3F and SMI 300 ion microscopes and the UC-HRL scanning ion microprobe.
Carbon 14 distribution images obtained by this method show that deoxyadenosine U-C14 is present in the cytoplasm as well as the nucleus, with a higher concentration in' the nucleoli. Our study clearly demonstrates that ion microscopy is well suited for carbon 14 detection and localization at the subcellular level, permitting a wide variety of microanalytical tracer experiments.
Hindie, E.; Hallégot, P.; Chabala, J. M.; Thorne, N. A.; Coulomb, B.; Levi-Setti, R.; and Galle, P.
"Ion Microscopy: A New Approach for Subcellular Localization of Labelled Molecules,"
Scanning Microscopy: Vol. 2
, Article 1.
Available at: https://digitalcommons.usu.edu/microscopy/vol2/iss4/1