Visualization and Analysis Techniques for Three Dimensional Information Acquired by Confocal Microscopy

Authors

G. J. Brakenhoff, University of Amsterdam
H. T. M. van der Voort, University of Amsterdam
M. W. Baarslag, University of Amsterdam
B. Mans, University of Amsterdam
J. L. Oud, University of Amsterdam
R. Zwart, University of Amsterdam
R. van Driel, University of Amsterdam

Abstract

Confocal Scanning Laser Microscopy (CSLM) is particularly well suited for the acquisition of 3-dimensional data of microscopic objects. In the CSLM a specific volume in the object is sampled during the imaging process and the result is stored in a digital computer as a three-dimensional memory array. Optimal use of these data requires both the development of effective visual representations as well as analysis methods. In addition to the well known stereoscopic representation method a number of alternatives for various purposes are presented. When rendering in terms of solid-looking or semitransparent objects is required, an algorithm based on a simulated process of excitation and fluorescence is very suitable. Graphic techniques can be used to examine the 3-dimensional shape of surfaces. For (near-)real time applications a representation method should not require extensive previous data-processing or analysis.

From the very extensive field of 3-D image analysis two examples are given.

Recommended Citation

Brakenhoff, G. J.; van der Voort, H. T. M.; Baarslag, M. W.; Mans, B.; Oud, J. L.; Zwart, R.; and van Driel, R. (1988) "Visualization and Analysis Techniques for Three Dimensional Information Acquired by Confocal Microscopy," Scanning Microscopy: Vol. 2: No. 4, Article 2.
Available at: https://digitalcommons.usu.edu/microscopy/vol2/iss4/2